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Midstream Pregnancy Test Instructions

Start testing from 7 days past ovulation! Fertility2Family Midstream Pregnancy Tests are a fast, reliable and easy-to-use method of detecting pregnancy. Fertility2Family Midstream Pregnancy Tests detect the hCG (human Chorionic Gonadotropin) hormone, which the placenta produces after the fertilised egg adheres to the uterus wall.

pregnancy tests instructions
How many seconds do you dip a pregnancy test in urine?

How to Perform a Pregnancy Test?

1. Remove the Midstream Pregnancy Test from the foil wrapper.
2. Remove the cap to expose the absorbent tip, avoiding contact with the absorbent end.
3. Hold the Pregnancy Test with the thumb grip with the exposed absorbent tip pointing downward. Urinate onto the absorbent tip only until it is thoroughly wet.
4. Recap the device and lay the test stick flat with the window facing up.
5. Results may be observed after 1 minute. Do not interpret test results after 5 minutes.
6. Discard the test device after a single use.

Interpreting the Midstream Pregnancy tests Results.

Negative: If only one coloured line appears in the control area, the test is negative, and you can assume you are not pregnant.

Positive: If two coloured lines appear in the control and test areas, the midstream pregnancy test is positive, and you can assume you are pregnant.

Invalid: The test is invalid if no distinct pink-purple-coloured band is visible both in the test area and control area. It is recommended that you should re-test using a new test strip. If the problem persists, contact Fertility2Family.

Pregnancy Test results
Interpretation of results of our Midstream Pregnancy Test


Testing for Pregnancy FAQ

When can I start testing for pregnancy?

There is no need to wait until you have missed your period to begin testing. You can test as early as seven days past ovulation or 5-7 days before your expected period. When testing early, try to use first-morning urine.

How accurate is the Fertility2Family Home Pregnancy Test?

In laboratory studies, the Fertility2Family Midstream Pregnancy Tests have been proven to be more than 99% accurate. Our Australian Midstream Pregnancy Tests are sensitive to hCG – 10mIU/ml making them one of the most sensitive on the market.

What should I do if I get a positive pregnancy test?

You should make an appointment to see your doctor to confirm the pregnancy and begin prenatal care.

What should I do if I get a negative HPT result?

A negative result indicated that the hCG hormone was not detected in your urine, and you are unlikely pregnant. If your period is late, we suggest you re-test; if this test is also negative, we recommend contacting your doctor.

The Test Line is not as dark as the Control Line. Am I pregnant?

Yes, you are pregnant even if a very light test line is detected, unlike an ovulation test where the test line needs to be as dark or darker than the control line to be a positive result. A pregnancy test is positive if a second line contains pink dye.

How do I increase my chances of falling pregnant?

It would help if you determined when you are ovulating, as this is your most fertile window. Using Fertility2Family Ovulation Test Strips or our Basal Body Temperature (BBT) thermometer, you can determine your most fertile window and dramatically increase your chances of falling pregnant. You may also find our cheap, Sperm Friendly Lubricant helpful. Conceive Plus lubricant has been scientifically formulated to match fertile cervical liquid and sperm, enhance sexual activity, and increase the likelihood of conception.

Want more FAQs answered? Read our Early Pregnancy Tests: Frequently Asked Questions


Each pouch contains one Midstream Pregnancy Test and one desiccant.


Stored at normal room temperature (4-25⁰C) up to the expiration date.
The test midstream should be kept from direct sunlight, moisture and heat. Do not freeze. Shelf life is 18 months.


  • As with all diagnostic tests, a definitive diagnostic should not be based on a single test result. Reading the Pregnancy Test Strip instructions before using and having results checked by a doctor after evaluating all clinical and laboratory findings 
  • Read directions for use carefully before performing this home pregnancy test. Pay attention to the position of the test and control line.
  • Do not use it beyond the labelled expiry date.
  • Do not reuse the pregnancy test strip. Discard the product after a single use.
  • Do not touch the membrane located within the test panel.
  • Do not use it if the pouch is damaged or open.
  • The device should be used immediately after opening the pouch. Prolonged exposure to ambient humidity will cause product deterioration.
  • Treat urine samples and use devices as if they are potentially infectious. Avoid contact.
  • Discard the desiccant safely.

Medications and Pregnancy Tests

Please consult your healthcare professional if you are taking any medications.
Due to the high sensitivity of the Fertility2Family Midstream Pregnancy Tests (10mIU), detecting hCG in the urine from a menopausal or peri-menopausal woman may not indicate pregnancy. Still, it may represent the detection of pituitary hCG, and the positive interpretation requires repeat testing by a laboratory-based method to confirm pregnancy.

Chemical pregnancies and pregnancy tests

Due to the high sensitivity of Fertility2Family Midstream Pregnancy Tests (10mIU), detecting a pregnancy that may be non-viable if testing before the first day of the missed period (often called a chemical pregnancy) is possible.

The reliability of the result will increase by, or after, the first day of the missed period to establish the viability of the pregnancy.

Buy our Pregnancy Tests in packs of;

1 x Pregnancy Mid-Stream Tests

5 x Pregnancy Mid-Stream Tests

10 x Pregnancy Mid-Stream Tests

20 x Pregnancy Mid-Stream Tests

30 x Pregnancy Mid-Stream Tests

50 x Pregnancy Mid-Stream Tests

100 x Pregnancy Mid-Stream Tests


Midstream Pregnancy Test Instructions also available for download 

Read our customer reviews on our Pregnancy Strip tests, Midstream Pregnancy tests, Ovulation Strip tests, Midstream Ovulation testsBasal Body Thermometer (BBT)

Cheap Pregnancy tests
Our highly sensitive Midstream Pregnancy Test (HPT) will give you quick, reliable results without the fuss. Begin testing up to seven days before your period is due
Cheap Pregnancy test
Midstream pregnancy tests save you the fuss of fiddley strip tests and collections cups, making them less messy and easier to use. Simply hold the absorbent end in your urine stream for a few moments, then recap and await the results.

Fertility2Family only uses trusted & peer-reviewed sources to ensure our articles’ information is accurate and reliable.

Betz D, Fane K. (2023) Human chorionic gonadotropin – statpearls – NCBI bookshelf. Available at: (Accessed: 02 October 2023).

Department of Health & Human Services Australia (2003) Ovulation and fertility, Better Health Channel. Available at: (Accessed: 02 October 2023).

Healthdirect Australia (2022) About the placenta, – role and complications | Pregnancy Birth and Baby. Available at: (Accessed: 02 October 2023). (2024) Fertility2family basal ovulation thermometer Australia, Available at: (Accessed: 02 October 2023). (2024) Fertility2Family ovulation tests Australia, Available at: (Accessed: 02 October 2023). (2024) Fertility2family pregnancy tests Australia, Available at: (Accessed: 02 October 2023).

Supporting Women’s Health and Fertility (2022) SASMAR conceive plus Australia sperm friendly lubricant, – Supporting Women’s Health and Fertility. Available at: (Accessed: 02 October 2023).

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Evan Kurzyp

Evan is the founder of Fertility2Family and is passionate about fertility education & providing affordable products to help people in their fertility journey. Evan is a qualified Registered Nurse and has expertise in guiding & managing patients through their fertility journeys.

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