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Ovulation Test Strip Instructions

Key Facts

  • The Fertility2Family Ovulation Predictor Kit detects the Luteinizing Hormone (LH) surge, indicating the best time for conception.
  • Begin testing based on your menstrual cycle length, ideally starting 11 days after your first period.
  • Collect urine samples between 10 am and 8 pm, avoiding first-morning urine to ensure accurate results.
  • Interpret results by comparing the test line's color intensity to the control line; a darker test line indicates an LH surge.
  • Limit fluid intake before testing to avoid dilution of LH in urine, which can affect results.
  • Store the OPK at room temperature and use it within the expiration date for reliable results.

Detecting the LH surge is vital for identifying when your body is ready to release an egg. This brief spike in luteinising hormone has been rigorously validated by scientific research, ensuring our Fertility2Family Ovulation Test Strip accurately signals the start of your fertile window. With this clear hormonal indicator, you can plan intercourse at the optimal time for conception, supported by reliable, evidence-based results.

Ovulation Tests instructions
Ovulation Test Strips: How Do They Predict Fertility?

When to Begin Testing for Ovulation?

  • For a standard 28-day cycle: Start testing on Day 11, testing daily until you detect your LH surge.
  • For shorter cycles (e.g., 24 days): Begin testing on Day 8 to detect an earlier surge.
  • For longer cycles (e.g., 32 days): Start testing around Day 15 to match a later ovulation.
  • If your cycle is uncertain or irregular: Start testing on Day 11 and adjust based on your pattern. If cycles are consistently shorter than 21 days or longer than 38 days, consult your healthcare provider for personalised advice.

ovulation and menstrual cycle table
When to start testing using our Ovulation Test Instructions

Urine Collection for testing

  • Collect urine in a small urine collection cup .
  • Do not use a first-morning urine sample, as LH is normally made in your body early in the morning.
  • The best time to collect your urine is 10 am-8 pm.
  • Collect urine at approximately the same time each day.
  • Reduce liquid intake approximately 2 hours before collecting your urine, as a diluted sample can prevent the ovulation test from detecting your LH surge.

Ovulation Test Strip Instructions

1. Allow the sealed ovulation predictor kit pouch and your urine sample to reach room temperature (18-30 degrees). 2. Remove the OPK device from the sealed pouch. 3. Immerse the OPK in the urine collection cup with the arrow end pointing towards the urine. Do not immerse past the MAX (MAXIMUM) line. Take the strip out after 15 seconds and lay the strip flat on a clean, dry, non-absorbent surface.

Interpretation of The Ovulation Test R esults

While waiting for your results, you may notice a coloured solution moving across the windows. This is normal. Positive ovulation results may be observed in as short as 40 seconds. However, a complete reaction time of 10 minutes is required to confirm negative results. To determine your results, compare the colour intensity of the test line to the control line as follows: Negative Ovulation Test: (No LH surge) Only one colour band appears on the control region, or the test line is lighter than the control line. Positive OPK: (LH Surge) If two colour lines are visible and the test line is equal to or darker than the control line, you will likely ovulate in the next 12-48 hours. Invalid Ovulation Test: The test is invalid if a control line does not appear. The most likely reason is insufficient specimen volume or incorrect test performance. This can be caused by not holding the test strip in the urine for at least 5 seconds or dipping past the MAX line. Repeat the test with a new kit and use the Ovulation Test Instructions.

positive ovulation strip test
Interpretation of Results on Our Ovulation Test Strips

Ovulation Predictor Kit FAQs

The OPK Test Line is not as dark as the Control Line. Am I ovulating?

Unlike pregnancy tests , ovulation test strips are not considered positive unless the test line is as dark as the control line.

What should I do if I have a very light Test Line on my ovulation test?

This may mean you are coming up to or coming down from your LH surge. Getting a very light line on your test at any stage during your cycle is possible. It is only considered positive if it is as dark or darker than the control line. Read more about the signs of ovulation and when you are most fertile.

Will the amount of liquid I drink affect the result?

Yes, consuming a large amount of fluids before testing will dilute the hormone in your urine. It is recommended that you limit your fluid intake to bout two hours before the test.

How long will the OPK line remain visible?

For best results, ovulation Test results should be read at 5 – 10 minutes. A positive result will never disappear. The line’s colour may become darker, and a tinted background may appear after several hours. Some negative results may later display a faint test line due to evaporation from the testing zone. Therefore, you should not read the results after 10 minutes and discard the test once you have read the result.

When should you have intercourse after LH surge?

Ovulation is likely to occur within 12 to 48 hours. As this is your most fertile time , sexual intercourse is recommended if you are trying to conceive.

How accurate is the Fertility2Family Ovulation Test Strip?

The Fertility2Family Ovulation Test Strip has been proven to be more than 99% accurate in laboratory studies. Our OvulationStrip’s sensitiluteinising hormone (LH) is 25mIU/ml of urine.

I use a basal body temperature (BBT) thermometer to determine ovulation. Do Fertility2Family Ovulation Tests replace the need for BBT tracking?

The shift in BBT primarily indicates that ovulation has already occurred, whereas the Fertility2Family Ovulation Test Strip indicates that ovulation is about to occur. Thus, we recommend using these two methods in conjunction. You can buy your Basal Body Temperature Thermometer here .

How do I know if I am pregnant?

Complete a Fertility2Family Pregnancy Test. These tests are so sensitive that you can begin testing seven days before your period is due! You can buy Fertility2Family Pregnancy Tests here. Although testing can commence immediately after ovulation, pregnancy detection becomes more reliable on or after the first day of the missed period.

I received an ovulation-positive result and had intercourse during these fertile days, but I have not become pregnant. What shall I do?

Many factors can affect your ability to become pregnant. It can take normal, healthy couples many months to achieve a pregnancy. We recommend continuing the use of Fertility2Family Ovulation Tests, and if pregnancy is not achieved within 6 to 12 months, contact your doctor.

What type of container can I use to collect urine?

Any clean container can be used to collect urine. However, you can purchase our specific urine collection cups, which are great for use with LH predictor kits and home pregnancy tests.


Ovulation Test Instructions

CONTENTS

Each pouch contains one ovulation test strip and one desiccant.

STORAGE & STABILITY

Ovulation Tests are stored at normal room temperature (4-25⁰C) until expiration. The test (OPK) should be kept from direct sunlight, moisture, and heat. Do not freeze. The shelf life is 18 months.

WARNINGS & PRECAUTIONS

  • Consult a healthcare professional for an accurate diagnosis. Don’t rely solely on ovulation test results to assess your fertility.
  • Read and follow the ovulation kit instructions carefully before each use to correctly identify both the test and control lines.
  • Check the expiry date before use and do not use any ovulation predictor kit past its labelled expiration.
  • Use each LH predictor kit only once. Dispose of the device immediately after a single use to maintain accuracy.
  • Avoid touching the test strip’s membrane to prevent contamination that may affect LH surge detection.
  • Do not use the test if the kit pouch is damaged or has been opened previously to ensure reliable results.
  • Open and use the kit immediately after removing it from its pouch. Prolonged exposure to humidity can compromise test performance.
  • Treat all urine samples and used test strips as potentially infectious. Practice proper hygiene and dispose of them safely.
  • Follow disposal guidelines for the desiccant to ensure it is discarded safely and prevent accidental misuse.
  • If you are taking medications that could affect hormone levels, consult your healthcare provider before using the ovulation test.

    Buy our Ovulation Tests in packs of:

    10 x Ovulation Strip Tests 20 x Ovulation Strip Tests 30 x Ovulation Strip Tests 50 x Ovulation Strip Tests 100 x Ovulation Strip Tests 200 x Ovulation Strip Tests + 200 x Strip Test Collection Cups


    Ovulation Strip Tests Instructions also available for download Our Midstream Ovulation Test Instructions Read our customer reviews on our Pregnancy Strip tests , Midstream Pregnancy tests , Ovulation Strip tests , Midstream Ovulation tests & Basal Body Thermometer (BBT)


    cheap Ovulation Strip Tests
    Our Ovulation predictor kits work by allowing you to detect your monthly LH Surge – the sudden and dramatic increase in Luteinising Hormone (LH) present in your urine just before you ovulate. LH is the hormone that facilitates ovulation (the release of the egg). When you detect your LH surge with an OPK, you know you are at your most fertile.
    Test line progression
    Ovulation predictor kits detect the Luteinising Hormone (LH) in your body.
    A positive test is interpreted if the two colour lines are visible and the test line is equal to or darker than the control line; you will likely ovulate in the next 12-48 hours.

    Sources:
    Fertility2Family only uses trusted & peer-reviewed sources to ensure articles’ information is accurate and reliable. Australian Concept Infertility Medical Center Luteinisingeinizing hormone: Infertility treatment: Australian concept, ACIMC. Available at: https://acimc.org/luteinizing-hormone/ (Accessed: 03 October 2023). Cleveland Clinic (2022) Menstrual cycle (normal menstruation): Overview & Phases, Cleveland Clinic. Available at: https://my.clevelandclinic.org/health/articles/10132-menstrual-cycle (Accessed: 03 October 2023). Demir, A. et al. (2022) Identification of the LH surge by measuring intact and total immunoreactivity in urine for prediction of Ovulation Time, Hormones (Athens, Greece). Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9464748/#: (Accessed: 03 October 2023). ProductReview.com.au (2024) Fertility2family basal ovulation thermometer Australia, ProductReview.com.au. Available at: https://www.productreview.com.au/listings/fertility2family-basal-ovulation-thermometer (Accessed: 03 October 2023). ProductReview.com.au (2024) Fertility2Family ovulation tests Australia, ProductReview.com.au. Available at: https://www.productreview.com.au/listings/fertility2family-ovulation-tests (Accessed: 03 October 2023). ProductReview.com.au (2024) Fertility2family pregnancy tests Australia, ProductReview.com.au. Available at: https://www.productreview.com.au/listings/fertility2family-pregnancy-tests (Accessed: 03 October 2023). Therapeutic Goods Administration Australia (TGA) (2023) Fertility2family – clinical chemistry hormone ivds (317593), Therapeutic Goods Administration (TGA). Available at: https://www.tga.gov.au/resources/artg/317593 (Accessed: 03 October 2023). Wilcox, A.J., Dunson, D. and Baird, D.D. (2000) The timing o’ the ‘fertile ‘indow’ in the menstrual cycle: Day specific estimates from a prospective study, BMJ (Clinical research ed.). Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC27529/ (Accessed: 03 October 2023).

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    Evan Kurzyp

    Evan is the founder of Fertility2Family and is passionate about fertility education & providing affordable products to help people in their fertility journey. Evan is a qualified Registered Nurse and has expertise in guiding & managing patients through their fertility journeys.

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