13 min read
May 3, 2026
Pregnancy Test Strip Instructions
Written by
Fertility2Family Team
Medically reviewed by
Evan Kurzyp, RN (AHPRA), BSN, Master of Nursing
Fertility2Family pregnancy test strips are made for home urine testing after a missed period or when you need to check whether hCG is present. They are simple to use, but the result depends on three things: using a clean urine sample, dipping only to the MAX line, and reading the strip at the correct time.
A pregnancy test strip can show whether human chorionic gonadotropin, usually called hCG, was detected in that urine sample at that time. It cannot confirm how far along you are, whether a pregnancy is progressing normally, or whether pain or bleeding is safe.
This page explains how to use Fertility2Family pregnancy test strips after purchase, including when to test, how to collect urine, how far to dip the strip, how to read positive, negative and invalid results, and when to speak with a GP in Australia. Always read the label and follow the directions for use. These strips are for self-testing and in vitro diagnostic use only. They are not to be taken internally. You can also download the official Fertility2Family pregnancy test strip instructions PDF if you want a printable copy.
Quick Answers About Pregnancy Test Strips
How do you use a Fertility2Family pregnancy test strip?
Collect urine in a clean, dry container. Dip the arrow end of the strip into urine without passing the MAX line, remove it after 15 seconds, lay it flat on a clean, dry, non-absorbent surface, then read the result at 5 minutes.
When should I use a pregnancy test strip?
For the most reliable home result, use a pregnancy test strip from the first day of a missed period. If you test earlier and get a negative result, repeat the test in 48 hours using first morning urine.
How do I read a pregnancy test strip result?
A positive result shows two coloured lines, even if the test line is faint. A negative result shows one control line only. An invalid result has no control line. Read the strip at 5 minutes and ignore any line or background change after 10 minutes.
What Fertility2Family Pregnancy Test Strips Detect
Fertility2Family pregnancy test strips detect hCG in urine. hCG is made after implantation, when early pregnancy tissue begins sending hormone signals into the body. The level usually rises over time in early pregnancy, which is why testing after a missed period gives a clearer result than testing very early.
The official Fertility2Family pregnancy test strip instructions list sensitivity to hCG as 25 mIU/mL. This means the strip is designed to detect hCG at that threshold when used correctly. The result is qualitative. It can show positive, negative or invalid, but it does not measure the exact amount of hCG in urine.
A pregnancy test strip answers one narrow question: was hCG detected in this urine sample during the correct reading window? It does not confirm pregnancy location, gestational age, hCG trend, miscarriage risk, or the cause of pelvic pain or bleeding.
What a Result Can and Cannot Tell You
A positive pregnancy test strip means hCG was detected. In most situations, that is a strong reason to book a GP appointment and discuss pregnancy confirmation, dating, medicines, supplements and antenatal care options in Australia.
A negative pregnancy test strip means hCG was not detected at the time of testing. It may be accurate, but it can also happen if you tested too early, ovulated later than expected, used diluted urine, dipped the strip incorrectly, or read the result outside the correct time.
An invalid result means the control line did not appear. Do not rely on that result, even if a test line appears. Retest with a new strip and follow the instructions again.
Seek urgent medical care if you have severe abdominal or pelvic pain, one-sided pain, shoulder tip pain, fainting, dizziness, heavy bleeding, or feel very unwell. Do this regardless of whether the home test is positive, negative or unclear.
When to Take a Pregnancy Test Strip
The official Fertility2Family instructions recommend testing from the first day of a missed period for best accuracy. This gives hCG more time to rise after implantation and reduces the chance of an early false negative.
If your cycle is regular, use your expected period as the main guide. If your period usually arrives on day 28, testing from day 29 is more reliable than testing several days earlier.
If your cycle is irregular, testing by calendar date can be less reliable. Late ovulation can delay implantation and hCG rise. If your period is late and the result is negative, repeat the test in 48 hours using first morning urine.
If you tracked ovulation, use that information to avoid testing too early. Pregnancy tests detect hCG after implantation, not immediately after sex or ovulation. If timing is uncertain, waiting until the expected period is usually the clearer option.
First Morning Urine
First morning urine is usually the best sample when testing early, close to the expected period, or after a previous unclear result. It is often more concentrated because you have not been drinking fluid for several hours.
If your period is several days late, you may still get a clear result later in the day. Even then, avoid drinking large amounts of fluid before testing. Diluted urine can make hCG harder to detect.
Before You Start Testing
Set up everything before opening the pouch. You need a clean dry urine container, the sealed Fertility2Family pregnancy test strip, a flat non-absorbent surface, and a timer. Check the expiry date and make sure the foil pouch is sealed and undamaged.
Open the pouch only when you are ready to test. Use the strip straight away after opening because humidity can affect the test if it is left exposed. Keep the desiccant sachet away from children and pets, and do not ingest it.
How to Collect Urine for Pregnancy Test Strips
Use a small, clean, dry container. The container should be free from water, soap, cleaning residue, food residue or anything else that could affect the sample.
If you test often, Fertility2Family urine collection cups can make strip testing easier. A cup gives the strip enough urine contact and helps you avoid dipping past the MAX line.
Collect the sample before opening the foil pouch. This keeps the strip sealed until you are ready to use it.
How to Use Fertility2Family Pregnancy Test Strips
Remove the strip from the pouch. Hold the coloured end and avoid touching the absorbent end. The absorbent end is the part that enters the urine.
Immerse the strip vertically into the urine with the arrow end pointing down. Do not immerse past the MAX line. The MAX line marks the highest point the urine should reach.
Remove the strip after 15 seconds. Lay it flat on a clean, dry, non-absorbent surface. Do not hold it upright while the result develops, and do not place it on tissue, cloth or a wet surface.
Read the result at 5 minutes. Do not read the result after 10 minutes. Any line, shadow or background change after 10 minutes is not valid.
How to Read Pregnancy Test Strip Results
Negative result
A negative result shows one coloured line in the control area only. This means hCG was not detected at that time. If your period is late, repeat the test in 48 hours using first morning urine. If repeat tests stay negative and your period does not arrive, speak with your GP.
Positive result
A positive result shows two coloured lines. One line appears in the control area and one line appears in the test area. The test line does not need to be as dark as the control line. Any coloured test line within the correct reading window should be treated as positive.
Invalid result
An invalid result means the control line does not appear. The result is invalid even if a test line appears. Retest with a new strip. Check the pouch, expiry date, dipping depth, urine contact time and reading time.
Faint Lines, Evaporation Lines and Indents
A faint coloured test line within the correct reading window should be treated as positive. It may be faint because hCG is still low, especially near the expected period or soon after implantation.
An evaporation line is different. It usually appears after the reading window as urine dries on the strip. It may look grey, colourless or shadow-like. Do not use a line that appears after 10 minutes to decide whether the result is positive.
An indent line can also cause confusion. Some strips have a slight mark where the test line would appear. If there is no colour within the reading window, it should not be treated as positive.
Set a timer, read at 5 minutes, ignore changes after 10 minutes, and repeat in 48 hours if the result is unclear. Fertility2Family has separate guides on a faint line on a pregnancy test and evaporation lines on pregnancy tests if you need more examples.
Why a Result Might Not Match Your Symptoms
A false negative can happen when the strip shows negative even though pregnancy has started. The most common reason is testing before enough hCG is present in urine. Late ovulation, diluted urine, incorrect dipping, damaged packaging, expired tests, and reading the result outside the correct time can also affect reliability. If this is your situation, read Fertility2Family’s guide to a false negative pregnancy test.
A false positive is less common, but it can happen. Medicines containing hCG, including some fertility treatment medicines or trigger injections, can affect results. A recent pregnancy, miscarriage, abortion or birth can also leave hCG in the body for a period of time. If the result does not fit your timing, symptoms or recent treatment history, your GP can arrange a blood hCG test and explain what the result means.
Home pregnancy tests should not be used to explain pain, heavy bleeding or feeling unwell. Symptoms matter, even when the test result seems clear.
Storage and Stability
Store Fertility2Family pregnancy test strips between 4 and 25 degrees Celsius until the expiry date. Keep each strip in the sealed pouch until use. Protect the strips from sunlight, moisture and heat. Do not freeze them.
Avoid storing tests in bathrooms, where steam and humidity can damage packaging. A cool dry cupboard is usually more suitable.
Each pouch contains one strip and one desiccant sachet. Do not ingest the desiccant. Keep tests and desiccant sachets out of reach of children and pets.
Disposal and Safety Precautions
Each strip is single use only. Do not reuse a pregnancy test strip. Once the strip has reacted, it cannot be used again.
Do not touch the membrane in the test area. Use the strip immediately after opening the pouch. Treat urine samples and used strips as potentially infectious. Avoid contact with the used test area, wash your hands after testing, and place the used strip in household waste. Do not flush it.
If you are using fertility medicines, hormone treatment, or medicines that may affect hCG, ask your healthcare professional when to test.
When to Speak With a GP in Australia
Book a GP appointment after a positive home pregnancy test. Your GP can discuss confirmation if needed, pregnancy dating, medicines, supplements and antenatal care options.
Speak with your GP if your period is late and repeated tests are negative, if your cycles are irregular and you are unsure when to test, or if symptoms do not match your result.
Seek urgent medical care if you have severe abdominal or pelvic pain, one-sided pain, shoulder tip pain, fainting, dizziness, heavy bleeding, or feel very unwell.
If you are trying to conceive and pregnancy has not occurred after 12 months under age 35, or after 6 months at age 35 or older, speak with your GP about fertility assessment.
Choosing the Right Fertility2Family Testing Product
The main product for this instruction page is Fertility2Family pregnancy test strips. If this is the product you already have, stay with the strip instructions on this page because the steps are specific to collected urine and dip-strip testing.
Pregnancy test strips can suit repeated testing near your expected period, especially if you prefer a compact format and are comfortable using a clean urine sample. If you want to test directly in the urine stream instead, the pregnancy tests category includes both strip and midstream formats.
The best format is the one you can use correctly. A strip test used exactly as directed is more useful than any test used outside its instructions.
Frequently Asked Questions About Pregnancy Test Strips Australia
Can PCOS affect pregnancy test strip results?
PCOS does not usually affect the hCG chemistry of a pregnancy test, but it can make ovulation and period timing less predictable. This can make it easier to test too early.
Can fertility medicines affect pregnancy test strip results?
Medicines containing hCG can affect results. This includes some fertility treatments and trigger injections. If this applies to you, follow your clinic’s advice on when to test.
Can a pregnancy test strip tell me how many weeks pregnant I am?
No. A pregnancy test strip gives a positive, negative or invalid result. It does not measure exact hCG or confirm gestational age.
Should the test line get darker each day?
The test line may become darker as hCG rises, but pregnancy test strips are not designed to measure exact hormone levels. If you need to know whether hCG is rising as expected, speak with your GP about blood testing.
What should I do if I bleed after a positive pregnancy test?
Light bleeding can happen in early pregnancy, but heavy bleeding, severe pain, shoulder tip pain, dizziness or fainting needs urgent medical care. Speak with your GP if any bleeding worries you.
Can I take a photo of my pregnancy test result?
Yes, but take the photo within the correct reading window. A photo taken at 5 minutes can help you compare or show your GP. Do not rely on photos taken after 10 minutes because late changes are not valid.
Next Steps If You Are Using Pregnancy Test Strips
Use a clean dry container, dip the strip for 15 seconds without passing the MAX line, lay it flat, read at 5 minutes, and ignore any changes after 10 minutes. A coloured second line within the reading window means hCG was detected. If the result is negative but your period is late, repeat the test in 48 hours using first morning urine. If you have pain, heavy bleeding, fainting, dizziness, or a result that does not match your symptoms, speak with your GP or seek urgent care.
Last reviewed: May 2, 2026
Next scheduled review: Mar 2028
References
Fertility2Family publishes Australia-focused fertility education. Articles are written by our team and medically reviewed by Australian-registered health practitioners. We use Australian consumer medicine information, Australian clinical and public health guidance, and peer-reviewed research consistent with Australian care. We explain what the evidence suggests, what it cannot confirm, and when to see a GP or fertility specialist. Each article lists its author, medical reviewer, and review dates.
https://fertility2family.com.au/wp-content/uploads/2021/05/F2F_Pregnancy-Test-Strip-Instructions.pdf
https://www.healthdirect.gov.au/pregnancy-tests
https://www.healthdirect.gov.au/hcg-test
https://www.healthdirect.gov.au/ectopic-pregnancy
https://www.pregnancybirthbaby.org.au/pregnancy-tests
https://www.betterhealth.vic.gov.au/health/healthyliving/pregnancy-testing
https://www.racgp.org.au/afp/2014/may/early-pregnancy-bleeding